Abstract
A relationship was established between estradiol 16 alpha-hydroxylation, papillomavirus and its malignant sequelae. In this study, we determined that 16 alpha-hydroxylation was substantial in normal larynx and further increased in laryngeal papillomas. Estradiol or 16 alpha-hydroxyestrone stimulated proliferation in both kinds of cells while 2-hydroxyestrone was antiproliferative. Indole-3-carbinol, which induces 2-hydroxylation, abrogated the proliferative effects of estradiol. In immunocompromised mice, papilloma cysts formed in HPV-11 infected laryngeal tissue implanted under the renal capsules in 100% of control mice but only in 25% of mice fed indole-3-carbinol.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anticarcinogenic Agents / therapeutic use*
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Aryl Hydrocarbon Hydroxylases*
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Cell Division / drug effects
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Cytochrome P-450 CYP2C8
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Cytochrome P-450 CYP2C9
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Estrogen Antagonists / pharmacology
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Estrogen Antagonists / therapeutic use*
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Estrogens / metabolism*
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Female
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Humans
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Hydroxyestrones / pharmacology
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Hydroxylation / drug effects
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Indoles / therapeutic use*
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Laryngeal Neoplasms / metabolism*
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Laryngeal Neoplasms / microbiology
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Laryngeal Neoplasms / prevention & control*
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Larynx / drug effects
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Larynx / metabolism
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Male
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Mice
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Mice, Inbred Strains
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Mice, Nude
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Mixed Function Oxygenases / metabolism
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Neoplasms, Hormone-Dependent / metabolism*
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Neoplasms, Hormone-Dependent / microbiology
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Neoplasms, Hormone-Dependent / prevention & control*
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Papilloma / immunology
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Papilloma / metabolism*
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Papilloma / prevention & control
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Papillomaviridae*
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Steroid 16-alpha-Hydroxylase
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Transplantation, Heterologous
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Tumor Virus Infections / complications*
Substances
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Anticarcinogenic Agents
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Estrogen Antagonists
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Estrogens
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Hydroxyestrones
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Indoles
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16-hydroxyestrone
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indole-3-carbinol
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Mixed Function Oxygenases
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CYP2C9 protein, human
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Cytochrome P-450 CYP2C9
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Aryl Hydrocarbon Hydroxylases
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CYP2C8 protein, human
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Cytochrome P-450 CYP2C8
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Steroid 16-alpha-Hydroxylase