Ampullarius canaliculatus is a large fresh-water snail of which the original habitat is said to be Argentina. Recently, the snail which grew wild has propagated in various parts of Japan, and is causing damage to aquatic plants by feeding on them. The present study was performed to find out whether the snail can be a suitable intermediate host for Angiostrongylus cantonensis. Natural infection with A. cantonensis was confirmed in the snails collected from 5 different places in 4 hamlets in Okinawa and in those from Ishigaki Island. All snails experimentally infected with first-stage larvae of the parasite showed second-stage and third-stage larvae developing at the time of examination later on. Thus, A. canaliculatus is a suitable intermediate host for A. cantonensis, and, if eaten raw, it can be a potential source of human infection with eosinophilic meningoencephalitis in the endemic areas.