Nucleotide sequence diversity of the bexA gene in serotypeable Haemophilus influenzae strains recovered from invasive disease patients in Canada

Jianwei Zhou; Dennis K S Law; Michelle L Sill; Raymond S W Tsang

doi:10.1128/JCM.00612-07

Nucleotide sequence diversity of the bexA gene in serotypeable Haemophilus influenzae strains recovered from invasive disease patients in Canada

J Clin Microbiol. 2007 Jun;45(6):1996-9. doi: 10.1128/JCM.00612-07. Epub 2007 Apr 25.

Authors

Jianwei Zhou¹, Dennis K S Law, Michelle L Sill, Raymond S W Tsang

Affiliation

¹ Vaccine Preventable Bacteria Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada R3E 32R2.

Abstract

The bexA genes of 36 Haemophilus influenzae isolates were sequenced to reveal their nucleotide sequence diversity, which divided them into two groups, similar to clonal divisions I and II. This sequence diversity may lead to false-negative PCR results for H. influenzae infections if bexA is the chosen gene target.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ATP-Binding Cassette Transporters / genetics*
Bacterial Proteins / genetics*
Base Sequence*
Canada
False Negative Reactions
Genetic Variation*
Haemophilus Infections / microbiology*
Haemophilus influenzae / classification*
Haemophilus influenzae / genetics
Haemophilus influenzae / isolation & purification
Humans
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction / methods
Sequence Analysis, DNA
Serotyping

Substances

ATP-Binding Cassette Transporters
Bacterial Proteins
bexA protein, Haemophilus influenzae

Associated data

GENBANK/EF490494
GENBANK/EF490495
GENBANK/EF490496
GENBANK/EF490497
GENBANK/EF490498
GENBANK/EF490499
GENBANK/EF490500
GENBANK/EF490501