Aph(3')-IIc, an aminoglycoside resistance determinant from Stenotrophomonas maltophilia

Aki Okazaki; Matthew B Avison

doi:10.1128/AAC.00795-06

Aph(3')-IIc, an aminoglycoside resistance determinant from Stenotrophomonas maltophilia

Antimicrob Agents Chemother. 2007 Jan;51(1):359-60. doi: 10.1128/AAC.00795-06. Epub 2006 Nov 6.

Authors

Aki Okazaki¹, Matthew B Avison

Affiliation

¹ Department of Cellular and Molecular Medicine, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom.

Abstract

We report the characterization of an intrinsic, chromosomally carried aph(3')-IIc gene from Stenotrophomonas maltophilia clinical isolate K279a, encoding an aminoglycoside phosphotransferase enzyme that significantly increases MICs of kanamycin, neomycin, butirosin, and paromomycin when expressed in Escherichia coli. Disruption of aph(3')-IIc in K279a results in decreased MICs of these drugs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aminoglycosides / pharmacology*
Anti-Bacterial Agents / pharmacology
Butirosin Sulfate / pharmacology
Drug Resistance, Multiple, Bacterial / genetics*
Escherichia coli / drug effects
Escherichia coli / genetics
Gram-Negative Bacterial Infections / microbiology
Humans
Kanamycin / pharmacology
Kanamycin Kinase / genetics*
Microbial Sensitivity Tests
Mutation
Neomycin / pharmacology
Stenotrophomonas maltophilia / drug effects*
Stenotrophomonas maltophilia / genetics

Substances

Aminoglycosides
Anti-Bacterial Agents
Butirosin Sulfate
Kanamycin
Kanamycin Kinase
Neomycin