New TEM variant (TEM-92) produced by Proteus mirabilis and Providencia stuartii isolates

Antimicrob Agents Chemother. 2001 Apr;45(4):1278-80. doi: 10.1128/AAC.45.4.1278-1280.2001.

Abstract

The sequences of the bla(TEM) genes encoding TEM-92 in Proteus mirabilis and Providencia stuartii isolates were determined and were found to be identical. Except for positions 218 (Lys-6) and 512 (Lys-104), the nucleotide sequence of bla(TEM-92) was identical to that of bla(TEM-20), including the sequence of the promoter region harboring a 135-bp deletion combined with a G-162-->T substitution. The deduced amino acid sequence of TEM-92 differed from that of TEM-52 by the presence of a substitution (Gln-6-->Lys) in the peptide signal.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution
  • Enterobacteriaceae Infections / drug therapy
  • Enterobacteriaceae Infections / microbiology
  • Genes, Bacterial
  • Humans
  • Microbial Sensitivity Tests
  • Promoter Regions, Genetic
  • Proteus Infections / drug therapy
  • Proteus Infections / microbiology
  • Proteus mirabilis / drug effects*
  • Proteus mirabilis / enzymology*
  • Proteus mirabilis / genetics
  • Providencia / drug effects*
  • Providencia / enzymology*
  • Providencia / genetics
  • Sequence Analysis, DNA
  • beta-Lactam Resistance
  • beta-Lactamases / genetics*

Substances

  • beta-lactamase TEM-92
  • beta-Lactamases