|
Jules Bordet and the Discovery of Bordetella pertussis By Janet Lee In
1900, Jules Bordet along with Octave Gengou observed a small ovoid bacterium in
the sputum of a 5-month old child suffering from pertussis, or whooping cough.
The bacterium was similar to the Haemophilus influenza, but showed
distinct morphological characteristics, which led Bordet and Gengou to consider
it as a separate species. It seemed that the elusive pathogen responsible for
whooping cough, a disease that was first described by Guillaume de Baillou in
1578, was finally identified. However, the discovery remained questionable, as
the organism was unable to be isolated and cultivated on ordinary ascitic agar
or blood agar plates.
Six years later, Bordet and Gengou succeeded in making a special medium, called Bordet-Gengou (BG) medium, which served as a key factor for isolating the difficult pathogen. BG medium was made from a melted fluid consisting of a glycerin extract of potato, agar, and salt solution, to which an equal volume of defibrinated rabbit or human blood was added. BG medium promoted the growth of colonies when inoculated with sputum taken preferably from the first day of the disease, a very short timeframe during which the microbe could be isolated. Other media and PCR have supplanted BG media today. The first pertussis bacillus was successfully isolated from the expectorate of Bordet’s own son, Paul, who had just contracted whooping cough.
Bordet and Gengou observed the isolated microbe in both biological samples and artificial BG medium. Biological samples showed a variable distribution of the microbe. However, a more striking appearance was seen on BG medium, as the scientists observed the bacterium to have a grayish glistening appearance that was reminiscent of drops of mercury. Other forms of the bacterium, such as the white nonhemolytic form, were found after subcultures in vitro. Unlike the hemolytic bacteria freshly isolated from patients, the nonhemolytic bacteria were not agglutinated by infected patient serum.
References
http://jrd.consultantlive.com/display/article/1145425/1404732 bl&ots=8iqAfeZJpi&sig=4ePZ7gxevRtA2tQZqFWmfmo1jUo&hl=en&ei=2_SVTqj2NqL00gGk6ozBBw&sa=X&oi=
book_result&ct=result&resnum=5&ved=0CDcQ6AEwBDgK#v=onepage&q=bordet%20discovery%20pertussis&f=false
pg. 535-538
http://www.discoveriesinmedicine.com/Com-En/DPT-Vaccine.html
http://cid.oxfordjournals.org/content/49/10/1565.full
|
|