Table 1.   In vitro activity of caspofungin, micafungin and anidulafungin against Candida sp. (9, 22, 28, 29, 59, 63, 65, 75, 76, 97)

 

Caspofungin

Micafungin

Anidulafungin

Species

N

MIC (Range)

MIC90

n

MIC (Range)

MIC90

n

MIC (Range)

MIC90

Candida albicans

3450

0.007->0.8

0.125-0.5

45

<0.0039-0.0625

0.0156-0.0625

42

<0.001-0.015

0.0078

Candida glabrata

756

0.007->0.8

0.25-1

39

0.0078-0.0625

0.0156-0.0039

13

0.0039-0.03

0.03

Candida krusei

185

0.125-2

1

19

0.125-0.25

0.125-0.25

11

0.0078-0.015

0.015

Candida parapsilosis

635

0.03->16

1-4

29

0.5-2

1

12

0.125-16

1

Candida tropicalis

127

0.03-2

0.125-1

13

0.0156-0.125

0.0313-0.125

10

0.002-0.015

0.0015

Candida lusitaniae

35

0.12-2

1-2

 

 

 

3

0.0015

0.0015

MIC = minimum inhibitory concentration (µg/ml); MIC90, = MIC required to inhibit 90% of organisms compared to growth control.

MICs were determined by broth microdilution with RPMI-1640 medium after incubation for 48 hours.

 

 

Table 2. In vitro activity of caspofungin, micafungin and anidulafungin against Aspergillus sp. (8, 30, 31, 70, 83, 90, 97)  

 

Caspofungin

Micafungin

Anidulafungin

Species

n

MIC90

n

MEC (Range)

n

MIC (Range)

MIC90

n

MIC90

Aspergillus flavus

97

0.12->32

58

<0.03-2

14

0.0078-0.0156

0.0156

9

<0.03

Aspergillus fumigatus

274

0.25->32

191

<0.03->8

47

0.0078-0.0156

0.0078-0.0156

28

<0.03

Aspergillus niger

30

0.25-1

39

<0.03-1

16

<0.0039-0.0156

0.0078

9

<0.03

Aspergillus terreus

36

0.2-0.6

26

<0.03-0.5

8

<0.0039-0.0078

0.0078

2

<0.03

MIC = minimum inhibitory concentration (µg/ml);  MIC90, = MIC required to inhibit 90% of organisms compared to growth controls.

MEC = minimal effective concentration

MICs were determined by broth microdilution with RPMI-1640 medium after incubation for 48 hours.

 

 

Table 3.  Pharmacokinetic parameters of caspofungin, micafungin and anidulafungin (16, 27, 34, 48, 79, 95, 96, 102, 103, 105)

 

Caspofungin

Micafungin

Anidulafungin

Plasma elimination half-life

9-11 h

11.6-15.2 h

29 h

30 h*

Volume of distribution

9.67L

14L

41.2L

Plasma protein binding

Extensive (97%)

Extensive (99%)

84%

Metabolism

Hepatic

(primarily hydrolysis to inactive metabolites)

Hepatic

Hepatic

Clearance

12 mL/min

13 mL/min

16 mL/min

* After oral dosing

 

 

Figure 1.  Structure of (a) Caspofungin, (b) Micafungin, and (c) Anidulafungin.

 

 

Figure 2. Effects of in vitro echinocandin exposure to (a) Candida species, (b) Aspergillus species. While the drugs typically kill Candida species, potentially resulting in fungicidal’ effects, activity against Aspergillus species is only appreciated in growing cells located at branch points and tips of filaments. This latter effect causes ballooning, blunting, and other forms of aberrant growth upon microscopic evaluation (b).